How to run command on mac shown in gray boxes online

Select Gels РІвЂ“В·РІвЂ вЂLabel Peaks to label each measured peak with its size as a percent of the total size of the measured peaks.If necessary, scroll the image vertically by holding down the space bar and dragging. For each peak, measure the size by clicking inside the peak with the Wand ToolРІвЂ вЂ.To access to all the lanes, it may be necessary to scroll the image vertically using the Scrolling ToolРІвЂ вЂ (Hold down the space bar to temporarily switch to this tool). Note that you can hold Shift to constrain lines to be either horizontal or vertical. Use the Straight Line Selection ToolРІвЂ вЂ to draw base lines and/or drop lines so that each peak of interest defines a closed area (ImageJ will automatically switch to the Straight Line tool).Select Gels РІвЂ“В·РІвЂ вЂPlot Lanes ( 3РІР‚вЂ°) to generate the lane profile plots.Repeat the previous step for each remaining lane.The selected lane is outlined and labelled, and РІР‚ВLane n selectedРІР‚в„ў is displayed in the status bar. Move the rectangular selection right to the next lane (or down if the lanes are horizontal) and select Gels РІвЂ“В·РІвЂ вЂSelect Next Lane ( 2РІР‚вЂ°).Select Gels РІвЂ“В·РІвЂ вЂSelect First Lane ( 1РІР‚вЂ°) and the lane will be outlined and РІР‚ВLane 1 selectedРІР‚в„ў displayed in the status bar.This should be the left most lane if the lanes are vertical or the top lane if the lanes are horizontal. Use the rectangular selection tool to outline the first lane.

Note that a copy of the gel image with the lane outlines can be created at any point using the Image РІвЂ“В·Overlay РІвЂ“В· Flatten РІвЂ вЂ command.

For practice, refer to the video tutorial on the ImageJ wikipage and use the File РІвЂ“В· Open Samples РІвЂ“В·РІвЂ вЂGel sample image (1-D gel) to perform the following steps.

How to run command on mac shown in gray boxes online